The signaling pathway of MAPK Pmk1p and the maintenance of cellular integrity of the Schizosaccharomyces Pombe
DOI:
https://doi.org/10.37135/ee.004.04.03Keywords:
schizosaccharomyces, cell wall, biomarkersAbstract
Three cascades of MAPKs have been described about the Schizosaccharomyces pombe such as: the pheromone response with Spk1p as MAP kinase, the stress response in which Sty1p/Spc1p is MAPK, and the maintenance of cell integrity led by Pmk1p/Spm1p. The elimination of any of the kinases of the integrity path causes morphological alterations and multitabicated cells under stress conditions; suggesting a role in ionic homeostasis and cell wall biosynthesis. So, it was proposed to study the role of the MAPK Pmk1p signaling pathway in the maintenance of cell integrity in S.pombe. The organism mainly used was the fission yeast S. pombe and different molecular strains of Escherichia coli were used to carry out the molecular cloning work. Different techniques of molecular cloning, genetic methods, Western Blot and determination of the activity of Pmk1p under stress conditions were used. The most important conclusions were that the "sensors" "Mtl2p and Wsc1p" signal towards Rho1p but they are not "authentic" components of the cascade, and their mutants do not present the Vic phenotype (viable in the presence of immunosuppressant and chloride ion), characteristic of the mutants in the components of the cascade. Mtl2p and Wsc1p do not play an important role in signaling in response to osmotic stress and cell wall damage through the cellular integrity pathway of Pmk1p.
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